After the first successful prosecution of the offence, Lambeth Council is determined to stamp out spitting in public.
Last week, a man was fined £120 by the courts for spitting in the street after Lambeth issued him with a penalty notice under anti-littering laws.
The council said that the ruling means officers will now be quick to issue fixed penalty notices to those who also commit the offence.
Councillor Jennifer Brathwaite, Cabinet member for Environment & Sustainability, said: “Most people find spitting extremely unpleasant – both as an act and the mess it leaves on our streets.
“Offenders need to know that if our enforcement officers see anybody spitting in public, they will be facing a big fine.”